Study of Seminal Acid Phosphatase and Alkaline Phosphatase Level in Relation to Sperm Count in Teaching Hospital

Background: There are multiple diagnostic tests and procedures are available for diagnosis of infertility but semen analysis still is considered as most valuable diagnostic test for investigation of infertility.it is equally important that the semen analysis should be performed by experienced and expert person by proper technique. Biochemical analysis seminal fluid provide valuable information regarding functional status of main and accessory genital organs.the acid phosphatase and alkaline phosphatase level in seminal fluid is higher than blood but its relation to inferlity is still unidentified and its results are conflicting .so this study is carried out to know any relationship between seminal acid phosphatase and alkaline phosphatase level to sperm counts. Objective: This study is carried out to know any relationship between seminal acid phosphatase and alkaline phosphatase level to sperm counts. Materials and Methods: this study was carried out at Department of Pathology of Shree M. P. Shah Medical College, Jamnagar, Gujarat, India during the one and half year period from 1st april 2004 to 31st October 2005. 100 cases of male infertility patients were enrolled as study group visiting OPD, G. G. Hospital, Jamnagar, Gujarat, India. Sperm count is done by improved neubauer counting chamber. seminal acid phosphatase and alkaline phosphatase level is determined by method suggested by kind and king. Results & conclusion: out of 100 patients most of the patient is in 26-35 years of age group.there is highest amount of acid phosphatase levels in azoospermic patients while lowest in in patient with highest sperm counts.there is no any significant relationship between alkaline phosphatase level with sperm count while there is inverse relationship between acid phosphatase levels with sperm count.